Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
1987-8-26
pubmed:abstractText
Incubation of cultured cells under specific conditions induces a dramatic change in the actin organization: induction of intranuclear and/or cytoplasmic actin rods (actin paracrystal-like intracellular structures). We have found that cofilin, a 21-kDa actin-binding protein, is a component of these rods. Antibodies directed against cofilin labeled intranuclear actin rods induced in cells treated with dimethyl sulfoxide or exposed to heat shock and also labeled cytoplasmic actin rods induced in cells incubated in specific salt buffers. Moreover, we found that these actin rods are not stained with fluorescent phalloidin derivatives at all and appear to be right-handed helices, different from straight bundles of F-actin such as stress fibers. In vitro experiments revealed that cofilin and phalloidin compete with each other for binding to F-actin. Since cofilin and phalloidin have the ability to stoichiometrically bind actin molecule in the filament in vitro, the above results seem to suggest that cofilin directly binds to actin molecule in nearly an equimolar ratio in these rods. We call these rods "actin/cofilin rods."
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-1118014, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-291981, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-3030380, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-3086110, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-321230, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-338616, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-3519257, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-3900086, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-4008469, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-4055781, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-4096896, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-4254541, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6232264, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6280220, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6319166, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6366238, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6504045, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6509022, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6775963, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-6928695, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-7001475, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-7188610, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-7192637, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-7199054, http://linkedlifedata.com/resource/pubmed/commentcorrection/3474653-7200195
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5262-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Cofilin is a component of intranuclear and cytoplasmic actin rods induced in cultured cells.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't