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rdf:type |
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lifeskim:mentions |
umls-concept:C0009226,
umls-concept:C0013879,
umls-concept:C0017337,
umls-concept:C0018557,
umls-concept:C0020792,
umls-concept:C0030016,
umls-concept:C0040649,
umls-concept:C0086860,
umls-concept:C1514873,
umls-concept:C1533691,
umls-concept:C1546857,
umls-concept:C1556066,
umls-concept:C1619636
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pubmed:issue |
11
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pubmed:dateCreated |
1987-7-8
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pubmed:abstractText |
The 5'-flanking region of the gene for hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase) is shown to contain promoter sequences that drive transcription in vitro in the presence of a HeLa whole-cell extract. DNase I protection studies revealed at least six different regions within the 277-base-pair (bp) promoter that bind nuclear proteins and produce "footprints." The functional significance of these sequences was determined through transcriptional analysis of a series of substitution mutations that scrambled short sequences throughout this region. Two of the footprint sequences were crucial for transcription in vitro; one of these contains a match in 6 of 6 bp, with a sequence in the adenovirus type 2 major late promoter that is known to be required for transcription. Scrambling a 26-bp sequence in a third footprint led to a consistent 2-fold increase in transcription, suggesting that this sequence might be a site for negative regulation. These studies define three regions that play a role in regulating transcription of the gene for HMG-CoA reductase, a negatively regulated enzyme in the cholesterol biosynthetic pathway.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-2991285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-2993923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-3025588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-3530495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-3785199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-3860301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-4075392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-4075400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6088070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6225933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6313230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6863296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6933441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3473472-6995544
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3614-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3473472-Animals,
pubmed-meshheading:3473472-Base Sequence,
pubmed-meshheading:3473472-Cell Nucleus,
pubmed-meshheading:3473472-Cricetinae,
pubmed-meshheading:3473472-Genes,
pubmed-meshheading:3473472-HeLa Cells,
pubmed-meshheading:3473472-Humans,
pubmed-meshheading:3473472-Hydroxymethylglutaryl CoA Reductases,
pubmed-meshheading:3473472-Liver,
pubmed-meshheading:3473472-Male,
pubmed-meshheading:3473472-Mesocricetus,
pubmed-meshheading:3473472-Mutation,
pubmed-meshheading:3473472-Plasmids,
pubmed-meshheading:3473472-Promoter Regions, Genetic,
pubmed-meshheading:3473472-Transcription, Genetic
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pubmed:year |
1987
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pubmed:articleTitle |
Identification of promoter elements required for in vitro transcription of hamster 3-hydroxy-3-methylglutaryl coenzyme A reductase gene.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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