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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1986-10-30
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pubmed:abstractText |
A specific binding assay was developed that monitors the interaction of 125I-labeled microtubule-associated proteins (MAPs) with tubulin or its fragments bound to nitrocellulose membrane. To identify the tubulin-binding domains for MAPs we have examined the binding of rat brain 125I-labeled MAP2 or 125I-labeled tau factors to 60 peptides derived from porcine alpha- and beta-tubulin. MAP2 and tau factors specifically interacted with two peptides derived from the carboxyl-terminal region of beta-tubulin, which are located between positions 392-445 and 416-445. In addition, there is a distinct tau-binding site at the amino-terminal region of alpha-tubulin. tau factors but not MAP2 displayed strong interaction with a peptide derived from the amino-terminal domain of alpha-tubulin between positions 1 and 75. To narrow down the location of the beta-tubulin binding site that is common to MAP2 and tau factors, we have synthesized five peptides that are homologous to the corresponding sequence from the porcine or rat carboxyl-terminal region. Binding studies with the synthetic peptides suggest that amino acid residues 434-440 of beta-tubulin are crucial for the interaction of MAP2 and tau factors.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-1056738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-1057175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-2868892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-3753592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-3894367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-3935441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-3965457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-3968174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-426824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-4514990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-4733239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-5319951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6185336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6269058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6322969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6385005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6388633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6547721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6765171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-6945576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-7019911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-714168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-7204409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-7263687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-7464932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3463956-913395
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7162-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1986
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pubmed:articleTitle |
Common and distinct tubulin binding sites for microtubule-associated proteins.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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