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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1986-9-25
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pubmed:abstractText |
Heat-sensitive immunoliposomes are capable of releasing the entrapped content at the target cell surface upon a brief heating to the phase transition temperature of the liposome membrane. In this study we have examined the delivery efficiency of drugs entrapped in heat-sensitive immunoliposomes. Immunoliposomes composed of dipalmitoyl phosphatidylcholine with entrapped [3H]uridine were incubated with target cells at 4 degrees C. The cell-liposome mixture was then heated to 41 degrees C and the uptake of [3H]uridine into the intracellular pool of phosphorylated uridine-containing molecules was measured. The immunoliposomes showed maximal release of the uridine at 41 degrees C, the phase transition temperature of dipalmitoyl phosphatidylcholine liposomes. The largest accumulation of [3H]uridine in the target cells also took place at 41 degrees C. The initial level of uptake of [3H]uridine released from immunoliposomes by heating was greatly enhanced over that observed for free [3H]uridine and [3H]uridine released from liposomes without attached antibody. The nucleoside uptake inhibitors nitrothiobenzylinosine, dipyridamole, and unlabeled uridine were able to inhibit uptake of [3H]uridine released from immunoliposomes. This supports the hypothesis that the enhanced uptake is due to a heat-induced release of [3H]uridine at the cell surface followed by transport and phosphorylation of [3H]uridine by the target cells. These results indicate the feasibility of using the heat-sensitive immunoliposomes as a target-specific drug delivery system.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-1159691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-13641241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-205355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-218628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-364652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-3911220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-3967009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-3978127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-4019583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-4027231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-432641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6210111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6313067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6358213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6572395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6615878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6722105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6811657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6892792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6893068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-6897439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-7050582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-7093307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3461478-7194141
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6117-21
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3461478-Animals,
pubmed-meshheading:3461478-Antibodies, Monoclonal,
pubmed-meshheading:3461478-Biological Transport,
pubmed-meshheading:3461478-Cell Line,
pubmed-meshheading:3461478-H-2 Antigens,
pubmed-meshheading:3461478-Hot Temperature,
pubmed-meshheading:3461478-Immunoglobulin G,
pubmed-meshheading:3461478-Kinetics,
pubmed-meshheading:3461478-Liposomes,
pubmed-meshheading:3461478-Phosphorylation,
pubmed-meshheading:3461478-Pulmonary Surfactants,
pubmed-meshheading:3461478-Uridine
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pubmed:year |
1986
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pubmed:articleTitle |
Enhanced delivery to target cells by heat-sensitive immunoliposomes.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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