Linked Life Data

3460694

Source:http://linkedlifedata.com/resource/pubmed/id/3460694

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1986-9-17
pubmed:abstractText
The dynamics in levels of HL-60 (a human promyelocytic leukemia cell line) c-myc RNA due to retinoic acid-induced myeloid differentiation were measured. An increase in levels of c-myc RNA occurred early in this process when the cells are known to be in a precommitment early regulatory state. The increased levels of c-myc RNA occurred before any G1-0-specific growth arrest or phenotypic differentiation. Cells in G1 and S had similar levels of c-myc RNA during this process. Onset of growth arrest and phenotypic differentiation preceded an apparent decline of c-myc RNA levels. Levels of c-myc RNA decreased only in advanced cultures with growth arrest and differentiation essentially completed. The kinetics and cell cycle dependence of the early increase in c-myc levels paralleled previously reported nuclear structural changes characteristic of the precommitment state. Since c-myc encodes a putative nuclear matrix protein, the results suggest a regulatory role for increased c-myc expression in mediating the nuclear structural change characteristic of precommitment early in the retinoic acid-induced process of HL-60 terminal myeloid differentiation. The results argue against a change in c-myc RNA levels as a requirement for G1 to S transit or for G1-0-specific growth arrest during terminal differentiation. In contrast, the results argue for a putative regulatory role for c-myc in induction of the precommitment early regulatory state. C-myc may thus act in a homeotic regulatory capacity during HL-60 terminal myeloid differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4156-61
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Increased c-myc RNA levels associated with the precommitment state during HL-60 myeloid differentiation.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't