Linked Life Data

3455730

Source:http://linkedlifedata.com/resource/pubmed/id/3455730

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1986-1-24
pubmed:abstractText
To facilitate the determination of the genomic location of the vaccinia virus gene(s) encoding alpha-amanitin resistance (alpha r) (Villarreal et al., J. Virol. 51:359-366, 1984), a collection of alpha r, temperature-sensitive (ts) mutants were isolated. The premise of these experiments was that mutants might be found whose dual phenotypes were the result of a single or two closely linked mutations. Genetic analyses of the alpha rts mutant library revealed two mutants, alpha rts7 and alpha rts12, that apparently fit this criterion; in alpha rts7 the two lesions were indistinguishable, whereas in alpha rts12 the two mutations were closely linked but separable. Cloned vaccinia virus HindIII DNA fragments were used to marker rescue the temperature-sensitive phenotype of these two dual mutants. The temperature-sensitive lesion of alpha rts7 was rescued by the HindIII N fragment (1.5 kilobases), whereas alpha rts12 was rescued by the neighboring HindIII M fragment (2.0 kilobases). The progeny virions of the alpha rts7 HindIII-N rescue reverted to an alpha-amanitin-sensitive phenotype, whereas the alpha rts12 HindIII-M progeny were still resistant to the drug. Taken together, these data indicate that the gene encoding alpha-amanitin resistance maps to the HindIII N fragment and provides evidence for the existence of essential vaccinia virus genes in a region of the genome previously believed to be nonessential for replication in tissue culture. Biochemical analyses revealed that both mutants were capable of synthesizing DNA as well as early and late viral proteins at the permissive and nonpermissive temperatures. At the nonpermissive temperature alpha rts12 and alpha rts7 were unable to process the major core precursors P94 and P65 into VP62 and VP60.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-107327, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-221909, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-226996, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-2981355, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-4204192, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-4737663, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-5652680, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6086946, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6262520, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6264687, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6281970, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6612992, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-6748162, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-7080448, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-7143565, http://linkedlifedata.com/resource/pubmed/commentcorrection/3455730-7269240
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-70
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Mapping the genomic location of the gene encoding alpha-amanitin resistance in vaccinia virus mutants.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.