Linked Life Data

3437475

Source:http://linkedlifedata.com/resource/pubmed/id/3437475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1988-4-1
pubmed:abstractText
Partial hemitransection of the rat nigrostriatal pathway has been applied to study changes in the expression of tubulin and actin cytoskeletal genes. RNA was isolated from ipsilateral and contralateral structures of substantia nigra and striatum tissues of lesioned or sham-operated animals by a combined proteinase K and oligo(dT)-cellulose affinity purification procedure. A significant increase in the oligo(dT)-cellulose-eluted RNA was observed in the lesioned substantia nigra and striatal tissues compared to naive controls. By 5 days, the RNA content of the lesioned nigra reached a value of 96-114.5 micrograms/mg DNA compared to 45.7-52.9 micrograms/mg DNA. Administration of GM1 gangliosides at daily intervals resulted by day 5 in a further increase (131.8-141.5 micrograms/mg DNA) in the RNA content of the lesioned but also of the sham-operated (81.9-97.8 micrograms/mg DNA) animals. By 18 hr, the lesioned nigra exhibited a four-fold increase in tubulin messenger RNA (mRNAtub) gene expression in comparison to the sham-operated animals. The substantia nigra tissue of GM1-treated animals exhibited a hybridization value for mRNAtub twice as high compared to GM1-untreated, lesioned animals. The effect of GM1 appeared selective for alpha-tubulin and beta-tubulin compared to actin transcript. The latter was also elevated in the lesioned nigra tissue above the naive or sham-operated animals. The data suggest that, after injury, cytoskeletal RNA transcripts are elevated and that GM1 may play a role in the regulation of their steady-state levels.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
615-20
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Selective enhancement of tubulin gene expression and increase in oligo(dT)-bound RNA in the rat brain after nigrostriatal pathway unilateral lesion and treatment with ganglioside.
pubmed:affiliation
Department of Neurobiology, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article