Linked Life Data

3434444

Source:http://linkedlifedata.com/resource/pubmed/id/3434444

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1988-2-26
pubmed:abstractText
Heparin facilities the acrosome reaction in bull and rabbit sperm. It binds to sperm saturably, and the binding affinity and the susceptibility to heparin are related to fertility. Isolation of heparin-binding proteins from washed ejaculated sperm reveals proteins mostly in the 14-18 kilodalton (kD) range. Seminal plasma inhibits heparin-induced capacitation, possibly by binding and inactivating heparin. These experiments focused on isolating and characterizing heparin-binding proteins in seminal plasma. Semen samples from 6 bulls were pooled, centrifuged twice for 10 min at 500 xg and finally at 2000 xg for 10 min. The supernatant was ultrafiltered on an Amicon YM-5 (5 kD cutoff) membrane against 40 mM Tris with 2 mM Ca2+ plus protease inhibitors. Five mg of protein were applied to a 1.5 x 10 cm column of heparin-Sepharose. Bound proteins were eluted with 200 ml of a 0 to 2 M NaCl gradient at 0.33 ml/min collecting 3 ml fractions. Two major peaks eluted at 0.12 and 0.46 M NaCl. Those were subjected to SDS-PAGE under reducing conditions. The 0.12 M peak was composed of small (mol wt 15.5 and 16.5 kD) polypeptides which were similar to those found on sperm. The 0.46 M peak included those polypeptides plus a 19.5 kD polypeptide and its trailing edge had a 29.5 kD polypeptide. These polypeptides may regulate capacitation by viture of their ability to bind heparin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0065-2598
pubmed:author
pubmed:issnType
Print
pubmed:volume
219
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
597-601
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Isolation and characterization of seminal fluid proteins that bind heparin.
pubmed:affiliation
Endocrinology-Reproductive Physiology Program, University of Wisconsin, Madison.
pubmed:publicationType
Journal Article