Linked Life Data

3431554

Source:http://linkedlifedata.com/resource/pubmed/id/3431554

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1988-3-3
pubmed:abstractText
The divalent cation Zn2+ enhanced the lysis of sheep erythrocytes (ShE) by guinea pig C4-deficient (C4D) serum. Enhancement by Zn2+ (0.1 mM) was demonstrated in the presence of cobra venom factor (CoVF, 2 units/ml) and optimal Mg2+ (1.0 mM). Moreover, Zn2+ (0.1 mM) in the presence of Mg2+ (1.0 mM) caused the lysis of ShE by C4D serum without the addition of CoVF. Further studies were therefore performed in the absence of CoVF. Although addition of Zn2+ or Mg2+ alone to some pools of C4D serum caused lysis of ShE, the greatest extent of lysis occurred when Zn2+ and Mg2+ were both added to the reaction mixtures. When C4D serum was resin-treated, both Mg2+ and Zn2+ were required for lysis. Addition of Ca2+ had no effect. This pathway required high concns of serum and was inhibited by pretreating the serum with zymosan (2 mg/ml serum, 37 degrees C, 1 hr) or by heating the serum at 56 degrees C for 20 min. K76-COOH monocarboxylic acid also inhibited this pathway. These results suggest that Zn2+ together with Mg2+ activates the alternative pathway on the nonactivator surface (ShE).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1345-50
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Effect of zinc on lysis of sheep erythrocytes by guinea pig C4-deficient serum.
pubmed:affiliation
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
pubmed:publicationType
Journal Article