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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-3-15
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pubmed:abstractText |
The effect of background adaptation on the steady-state levels of alpha-melanotropin in the pars intermedia of the larval amphibian. Ambystoma tigrinum, was investigated. Acid extracts of pars intermedia obtained from light-adapted and dark-adapted animals were analyzed by radioimmunoassay following Sephadex gel filtration chromatography, reverse-phase HPLC, and Sulfopropyl Sephadex cation-ion-exchange chromatography. For both background adaptation conditions similar results were obtained. The major form of alpha-melanotropin present in the pars intermedia has the following properties: (1) an apparent molecular mass of 1.5 kDa; (2) a net charge at pH 3.5 of +4; and (3) a retention time following reverse-phase HPLC similar to that of synthetic ACTH(1-13)amide. In dark-adapted animals a minor form of alpha-melanotropin which has a net charge of +3 at pH 3.5 was also detected. The latter form represented approximately 10% of the total alpha-melanotropin immunoreactivity in the pars intermedia of dark-adapted animals. These results strongly suggest that the predominant form of alpha-melanotropin in the pars intermedia of larval A. tigrinum is a nonacetylated ACTH(1-13)amide-like polypeptide.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0016-6480
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-30
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3428562-Adaptation, Physiological,
pubmed-meshheading:3428562-Ambystoma,
pubmed-meshheading:3428562-Animals,
pubmed-meshheading:3428562-Brain Chemistry,
pubmed-meshheading:3428562-Chromatography, Gel,
pubmed-meshheading:3428562-Chromatography, High Pressure Liquid,
pubmed-meshheading:3428562-Chromatography, Ion Exchange,
pubmed-meshheading:3428562-Larva,
pubmed-meshheading:3428562-Light,
pubmed-meshheading:3428562-Melanocyte-Stimulating Hormones
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pubmed:year |
1987
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pubmed:articleTitle |
Isolation of alpha-melanotropin from the pars intermedia of the larval amphibian, Ambystoma tigrinum.
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pubmed:affiliation |
University of Denver, Department of Biological Sciences, Colorado 80208.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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