Linked Life Data

3427782

Source:http://linkedlifedata.com/resource/pubmed/id/3427782

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
1988-3-2
pubmed:abstractText
Middle ear effusion was obtained from children with chronic secretory otitis media undergoing myringotomy. The effusions contained about 120 mg/ml non-dialysable solids, of which 18-31% was mucus glycoprotein. The purified mucus glycoprotein had a composition characteristic of other mucus glycoproteins. Amino acid analysis of the glycoprotein indicates a protein core consisting of glycosylated regions resistant to proteolysis and non-glycosylated regions susceptible to proteolysis. Analysis of the mucus glycoprotein by gel filtration on Sepharose 2B showed that reduction caused a decrease in hydrodynamic size and proteolysis caused a further decrease. The difference was confirmed by sedimentation coefficient and viscosity measurements. The reduced glycoprotein had an intrinsic viscosity of 0.113 ml/mg and an S0(20) of 15.2S compared to a value of 0.018 ml/mg and 9.6S for the proteolytically digested glycoprotein. These results suggest a model for this middle ear mucus glycoprotein, in which the native glycoprotein is a large molecular mass polymer maintained by disulphide bridges. These disulphide linked glycoprotein units are broken down into smaller units by proteolysis. The mucus glycoprotein could not be purified completely free from low molecular mass components. A glycoprotein, susceptible to proteolysis Mr 28,000-33,000 co-fractionates with the major high molecular mass mucus glycoprotein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-8981
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
169
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-97
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Characterization of human middle ear mucus glycoprotein in chronic secretory otitis media (CSOM).
pubmed:affiliation
Department of Physiological Sciences, University of Newcastle upon Tyne, Medical School, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't