Linked Life Data

3422523

Source:http://linkedlifedata.com/resource/pubmed/id/3422523

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-3-11
pubmed:abstractText
Hepatitis B virus (HBV) contains an enhancer element that activates the viral core and X gene promoters. To investigate the transcriptional regulation of the viral S gene promoter, we transfected SK-Hep1 cells with circularized forms of HBV DNAs and their enhancerless mutants. We have found that expression of the S gene, determined by measurement of the appearance of HBsAg in the media and by RNA analysis, is to a large extent enhancer-dependent. This observation was further confirmed by analysis of a series of plasmids containing the chloramphenicol acetyl-transferase (CAT) gene under the control of the S gene promoter and the HBV enhancer element. Interestingly, in contrast to its behavior in SK-Hep1 cells, the S gene promoter is highly active in Alexander cells, in the absence of the enhancer element. This implies that activity of the S gene promoter is cell-type specific.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
162
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
362-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Regulation of hepatitis B virus S gene promoter in transfected cell lines.
pubmed:affiliation
Department of Virology, Weizmann Institute of Science, Rehovot, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't