Linked Life Data

3415963

Source:http://linkedlifedata.com/resource/pubmed/id/3415963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1988-10-18
pubmed:abstractText
Lipid vesicles with a positive surface charge have been prepared by using mixtures of phosphatidylethanolamine (PE) and phosphatidylcholine (PC) together with low mole fractions of a cationic lipid analogue, 1,2-bis(oleoyloxy)-3-(trimethylammonio)propane (DOTAP). We have used measurements of vesicle aggregation, lipid mixing, contents mixing, and contents leakage to examine the interactions between these vesicles and similar vesicles that carry a negative surface charge. Mixtures of vesicles with opposite surface charges aggregate readily at physiological or lower ionic strengths, and the extent of this aggregation is enhanced for vesicles that contain high proportions of PE relative to PC. Mixing of lipids and aqueous contents can also be observed between such vesicles, particularly when the vesicles contain substantial proportions of PE. Surprisingly, these latter processes are strongly promoted by monovalent salts and do not proceed at very low ionic strengths. PE/DOTAP vesicles show substantial lipid mixing with negatively charged vesicles containing high proportions of phosphatidylcholine, as well as with human erythrocyte ghosts, on a time scale of a few minutes. These interactions are strongly promoted both by the presence of high levels of PE in the cationic vesicles and by the presence of complementary surface charges on the two membrane populations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3917-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Interactions of cationic lipid vesicles with negatively charged phospholipid vesicles and biological membranes.
pubmed:affiliation
Department of Biochemistry, McGill University, Montréal, Québec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't