| pubmed-article:3413083 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3413083 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
| pubmed-article:3413083 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:3413083 | lifeskim:mentions | umls-concept:C2266196 | lld:lifeskim |
| pubmed-article:3413083 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:3413083 | pubmed:dateCreated | 1988-10-5 | lld:pubmed |
| pubmed-article:3413083 | pubmed:abstractText | Recent studies have suggested that the canalicular bile salt transport system of rat liver corresponds to a 100-kDa membrane glycoprotein. In the present study we attempted to functionally reconstitute the 100-kDa protein into artificial proteoliposomes. Canalicular membrane proteins were solubilized with octyl glucoside in the presence of asolectin phospholipids. The extracts were treated with preimmune serum or the 100-kDa protein selectively immunoprecipitated with a polyclonal antiserum. Proteins remaining in the supernatant were then incorporated into proteoliposomes by gel-filtration chromatography. Canalicular proteoliposomes containing the 100-kDa protein exhibited transstimulatable taurocholate uptake that could be inhibited by 4,4'-diisothiocyanato-2,2'-stilbenedisulfonic acid (DIDS). In contrast, no DIDS-sensitive transstimulatable taurocholate uptake was found in 100-kDa protein-free canalicular proteoliposomes. However, when the immunoprecipitated 100-kDa protein was dissociated from the antibodies and exclusively incorporated into liposomes, reconstitution of DIDS-sensitive transstimulatable and electrogenic taurocholate anion transport was again positive. Although incorporation of solubilized basolateral membrane proteins into liposomes also resulted in a prompt reconstitution of Na+ gradient-driven taurocholate uptake, the anti-100-kDa antibodies had no effects on the reconstituted transport activity of basolateral proteins. Thus, the findings establish that the previously characterized canalicular-specific 100-kDa protein is directly involved in the transcanalicular secretion of bile salts. | lld:pubmed |
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| pubmed-article:3413083 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:3413083 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:3413083 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3413083 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:3413083 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:3413083 | pubmed:author | pubmed-author:MeierP JPJ | lld:pubmed |
| pubmed-article:3413083 | pubmed:author | pubmed-author:HugentoblerGG | lld:pubmed |
| pubmed-article:3413083 | pubmed:author | pubmed-author:RuetzSS | lld:pubmed |
| pubmed-article:3413083 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3413083 | pubmed:volume | 85 | lld:pubmed |
| pubmed-article:3413083 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3413083 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3413083 | pubmed:pagination | 6147-51 | lld:pubmed |
| pubmed-article:3413083 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:3413083 | pubmed:meshHeading | pubmed-meshheading:3413083-... | lld:pubmed |
| pubmed-article:3413083 | pubmed:year | 1988 | lld:pubmed |
| pubmed-article:3413083 | pubmed:articleTitle | Functional reconstitution of the canalicular bile salt transport system of rat liver. | lld:pubmed |
| pubmed-article:3413083 | pubmed:affiliation | Department of Medicine, University Hospital, Zurich, Switzerland. | lld:pubmed |
| pubmed-article:3413083 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:3413083 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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