Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1988-10-5
pubmed:abstractText
Recent studies have suggested that the canalicular bile salt transport system of rat liver corresponds to a 100-kDa membrane glycoprotein. In the present study we attempted to functionally reconstitute the 100-kDa protein into artificial proteoliposomes. Canalicular membrane proteins were solubilized with octyl glucoside in the presence of asolectin phospholipids. The extracts were treated with preimmune serum or the 100-kDa protein selectively immunoprecipitated with a polyclonal antiserum. Proteins remaining in the supernatant were then incorporated into proteoliposomes by gel-filtration chromatography. Canalicular proteoliposomes containing the 100-kDa protein exhibited transstimulatable taurocholate uptake that could be inhibited by 4,4'-diisothiocyanato-2,2'-stilbenedisulfonic acid (DIDS). In contrast, no DIDS-sensitive transstimulatable taurocholate uptake was found in 100-kDa protein-free canalicular proteoliposomes. However, when the immunoprecipitated 100-kDa protein was dissociated from the antibodies and exclusively incorporated into liposomes, reconstitution of DIDS-sensitive transstimulatable and electrogenic taurocholate anion transport was again positive. Although incorporation of solubilized basolateral membrane proteins into liposomes also resulted in a prompt reconstitution of Na+ gradient-driven taurocholate uptake, the anti-100-kDa antibodies had no effects on the reconstituted transport activity of basolateral proteins. Thus, the findings establish that the previously characterized canalicular-specific 100-kDa protein is directly involved in the transcanalicular secretion of bile salts.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-2440891, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-2864346, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-2988507, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-2990676, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3005284, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3007150, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3022600, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3092813, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3311027, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3312195, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3479775, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3542777, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3556579, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3593261, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3675867, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3778896, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-3897250, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6159805, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6163373, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6265640, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6344992, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6389550, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6469975, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6591186, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6630516, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6643579, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6691970, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6699096, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6707198, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6863316, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-6929523, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-7118069, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-7160376, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413083-7213617
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6147-51
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Functional reconstitution of the canalicular bile salt transport system of rat liver.
pubmed:affiliation
Department of Medicine, University Hospital, Zurich, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't