Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
1988-10-5
pubmed:abstractText
A wheat embryo cytokinin-binding protein was covalently modified with the radiolabeled photoaffinity ligand 2-azido-N6-[14C]benzyladenine. A single labeled peptide was obtained after proteolytic digestion and isolation by reversed-phase and anion-exchange HPLC. Sequencing by classical Edman degradation identified 11 of the 12 residues but failed to identify the labeled amino acid. Analysis by laser photodissociation Fourier-transform mass spectrometry of 10 pmol of the peptide independently confirmed the Edman data and also demonstrated that the histidine residue nearest the C terminus (underlined) was modified by the reagent in the sequence Ala-Phe-Leu-Gln-Pro-Ser-His-His-Asp-Ala-Asp-Glu.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-1147949, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16659772, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16660972, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16661016, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16661618, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16664477, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-3462691, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-3468502, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-382986, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-6525415, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-6847617, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-7437072, http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-909454
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5927-31
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry.
pubmed:affiliation
ARCO Plant Cell Research Institute, Dublin, CA 94568.
pubmed:publicationType
Journal Article