rdf:type |
|
lifeskim:mentions |
umls-concept:C0019602,
umls-concept:C0020792,
umls-concept:C0030956,
umls-concept:C0033684,
umls-concept:C0037813,
umls-concept:C0162801,
umls-concept:C0205171,
umls-concept:C0205409,
umls-concept:C1123020,
umls-concept:C1709915,
umls-concept:C1880022
|
pubmed:issue |
16
|
pubmed:dateCreated |
1988-10-5
|
pubmed:abstractText |
A wheat embryo cytokinin-binding protein was covalently modified with the radiolabeled photoaffinity ligand 2-azido-N6-[14C]benzyladenine. A single labeled peptide was obtained after proteolytic digestion and isolation by reversed-phase and anion-exchange HPLC. Sequencing by classical Edman degradation identified 11 of the 12 residues but failed to identify the labeled amino acid. Analysis by laser photodissociation Fourier-transform mass spectrometry of 10 pmol of the peptide independently confirmed the Edman data and also demonstrated that the histidine residue nearest the C terminus (underlined) was modified by the reagent in the sequence Ala-Phe-Leu-Gln-Pro-Ser-His-His-Asp-Ala-Asp-Glu.
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-1147949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16659772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16660972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16661016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16661618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-16664477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-3462691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-3468502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-382986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-6525415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-6847617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-7437072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3413067-909454
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pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Aug
|
pubmed:issn |
0027-8424
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:volume |
85
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
5927-31
|
pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:3413067-Adenine,
pubmed-meshheading:3413067-Affinity Labels,
pubmed-meshheading:3413067-Amino Acid Sequence,
pubmed-meshheading:3413067-Arabidopsis Proteins,
pubmed-meshheading:3413067-Binding Sites,
pubmed-meshheading:3413067-Carrier Proteins,
pubmed-meshheading:3413067-Cytokinins,
pubmed-meshheading:3413067-Histidine,
pubmed-meshheading:3413067-Kinetin,
pubmed-meshheading:3413067-Mass Spectrometry,
pubmed-meshheading:3413067-Plant Proteins
|
pubmed:year |
1988
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pubmed:articleTitle |
Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry.
|
pubmed:affiliation |
ARCO Plant Cell Research Institute, Dublin, CA 94568.
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pubmed:publicationType |
Journal Article
|