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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1978-3-10
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pubmed:abstractText |
Among temperature resistant revertants of a temperature sensitive E. Coli alanyl-tRNA synthetase mutant a strain was found which contains an alanyl-tRNA synthetase with an additional mutation in the structural gene of the enzyme. This mutant enzyme has a 9 or 38 fold decreased Km value for alanine compared to that of the thermolabile parental enzyme or to wild-type enzyme, respectively. The alaS gene maps just counterclockwise from recA on the E. coli map (94% cotransduction frequency). It appears that the enzyme's increased affinity for alanine is the mechanism of suppressing the temperature sensitive character of the cell. In addition, some cold-sensitive temperature resistant revertants were found, where the cold-sensitive character mapped near strA. Presumably they are due to changes in ribosomal proteins as characterized by Ruffler et al. (1974).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine-tRNA Ligase,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acyl-tRNA Synthetases,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
156
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
221-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:340903-Alanine,
pubmed-meshheading:340903-Alanine-tRNA Ligase,
pubmed-meshheading:340903-Amino Acids,
pubmed-meshheading:340903-Amino Acyl-tRNA Synthetases,
pubmed-meshheading:340903-Bacterial Proteins,
pubmed-meshheading:340903-Escherichia coli,
pubmed-meshheading:340903-Genes,
pubmed-meshheading:340903-Hot Temperature,
pubmed-meshheading:340903-Kinetics,
pubmed-meshheading:340903-Mutation,
pubmed-meshheading:340903-Suppression, Genetic
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pubmed:year |
1977
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pubmed:articleTitle |
Suppression of a defective alanyl-tRNA synthetase in Escherichia coli: a compensatory mutation to high alanine affinity.
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pubmed:publicationType |
Journal Article
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