Linked Life Data

3408731

Source:http://linkedlifedata.com/resource/pubmed/id/3408731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1988-10-11
pubmed:abstractText
Equilibrium Raman spectra show that A- and B-form phosphodiester backbone geometries are both present in the solution structure of the RNA.DNA hybrid poly(rA).poly(dT) and that these arise from C3'-endo-rA and C2'-endo-dT nucleosides, respectively. Raman dynamic measurement of deuterium exchange of adenine 8CH groups reveals (i) a single kinetic class of rA conformers and (ii) extraordinary retardation of 8CH exchange in this class--more than 100-fold slower than in canonical DNA structures. The equilibrium and kinetic results, in conjunction with model building, indicate an unusual intrastrand hydrogen bond involving adenosine donor (8C-H) and acceptor (5'O) groups and a double-helical conformation in solution similar to that proposed for fibers at high relative humidity [Zimmerman, S. B., & Pheiffer, B. H. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 78-82]. In fibers of poly(rA).poly(dT) at low relative humidity, the Raman spectra indicate a conventional A-helix structure.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3868-73
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
A solution structure for poly(rA).poly(dT) with different furanose pucker and backbone geometry in rA and dT strands and intrastrand hydrogen bonding of adenine 8CH.
pubmed:affiliation
Division of Cell Biology and Biophysics, School of Basic Life Sciences, University of Missouri, Kansas City 64110.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.