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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1988-9-22
|
| pubmed:abstractText |
The distribution of melanin-concentrating hormone-like immunoreactivity was investigated by radioimmunoassay in the CNS of rat, guinea-pig, pig and man. Highest concentrations of melanin-concentrating hormone-like immunoreactivity were found in the hypothalamus of all the species: rat 204.4 +/- 14.9; guinea-pig 159.5 +/- 23.3; pig 10.9 +/- 4.5 and man 80.1 +/- 19.1 pmol/g. Gel chromatographic analysis of hypothalamic extracts showed five immunoreactive peaks of melanin-concentrating hormone-like immunoreactivity in the rat and pig and six in the guinea-pig and man. High-performance liquid chromatography analysis of hypothalamic extracts showed five immunoreactive peaks in rat, guinea-pig, pig and four in man. However, these peaks appeared at different retention times from that of the single peak of salmon melanin-concentrating hormone. Examination of subcellular fractions of whole rat brain showed that most of the melanin-concentrating hormone-like immunoreactivity is found in the synaptosome fraction. Stimulation of melanin-concentrating hormone-like immunoreactivity release from rat hypothalamic slices revealed that potassium in the presence of calcium stimulated melanin-concentrating hormone-like immunoreactivity release. These findings suggest that mammalian melanin-concentrating hormone-like immunoreactivity has a different amino acid sequence from salmon melanin-concentrating hormone and may exist in multiple molecular forms. It is possible that melanin-concentrating hormone may play a role as a neurotransmitter or modulator in the mammalian CNS.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0306-4522
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
25
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
925-30
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:3405434-Adult,
pubmed-meshheading:3405434-Aged,
pubmed-meshheading:3405434-Aged, 80 and over,
pubmed-meshheading:3405434-Animals,
pubmed-meshheading:3405434-Brain,
pubmed-meshheading:3405434-Female,
pubmed-meshheading:3405434-Guinea Pigs,
pubmed-meshheading:3405434-Humans,
pubmed-meshheading:3405434-Hypothalamic Hormones,
pubmed-meshheading:3405434-Male,
pubmed-meshheading:3405434-Melanins,
pubmed-meshheading:3405434-Middle Aged,
pubmed-meshheading:3405434-Pituitary Hormones,
pubmed-meshheading:3405434-Radioimmunoassay,
pubmed-meshheading:3405434-Rats,
pubmed-meshheading:3405434-Rats, Inbred Strains,
pubmed-meshheading:3405434-Spinal Cord,
pubmed-meshheading:3405434-Subcellular Fractions
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
The distribution of melanin-concentrating hormone-like immunoreactivity in the central nervous system of rat, guinea-pig, pig and man.
|
| pubmed:affiliation |
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, U.K.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|