Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
24
pubmed:dateCreated
1988-9-20
pubmed:abstractText
We have studied the molecular composition of the nuclear lamina in rat tissues of distinct embryological origin and the occurrence of the nuclear lamins during in vitro differentiation of the mouse F9 teratocarcinoma cell line. Immunochemical analysis demonstrated that all rat tissues contained the three major lamin forms (lamins A, B, and C) previously recognized in rat liver nuclei; however, other minor cross-reactive components were also identified in some tissues. The amount of the 67-kDa lamin B complexed with lamins A and C in the laminae of different tissues ranged from a stoichiometry of much less than 1 to approximately 1. Furthermore, it was found that F9 stem cells and their differentiated progeny express only lamin B, and Northern blotting analysis indicated that these cells fail to accumulate lamin A and C mRNA. Chemical cleavages and peptide mapping suggested that the 67-kDa lamin B form was of similar primary structure in all differentiated tissues and F9 cells. Employing antibodies with different affinities for phosphorylated and nonphosphorylated lamin B, we showed that the apparent invariance in the expression of this polypeptide is overriden by a heterogeneity produced via tissue-specific phosphorylation. Because similar differences in antibody recognition could be reproduced in vitro by phosphorylating lamin B with protein kinase A, we have concluded that the tissue-specific modifications of this protein may occur at consensus sites recognized by this enzyme. These data support the hypotheses that the lamins can form functional laminae by associating at various combinations, and that processes including differential lamin synthesis and post-translational modification can produce a steady state lamina heterogeneity.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12135-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:3403563-Animals, pubmed-meshheading:3403563-Autoantibodies, pubmed-meshheading:3403563-Brain Chemistry, pubmed-meshheading:3403563-Cell Nucleus, pubmed-meshheading:3403563-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:3403563-Female, pubmed-meshheading:3403563-Gene Expression Regulation, pubmed-meshheading:3403563-Humans, pubmed-meshheading:3403563-Immunoassay, pubmed-meshheading:3403563-Kidney, pubmed-meshheading:3403563-Lamin Type A, pubmed-meshheading:3403563-Lamin Type B, pubmed-meshheading:3403563-Lamins, pubmed-meshheading:3403563-Liver, pubmed-meshheading:3403563-Molecular Weight, pubmed-meshheading:3403563-Nuclear Proteins, pubmed-meshheading:3403563-Nucleic Acid Hybridization, pubmed-meshheading:3403563-Peptide Fragments, pubmed-meshheading:3403563-Phosphorylation, pubmed-meshheading:3403563-RNA, Messenger, pubmed-meshheading:3403563-Rats, pubmed-meshheading:3403563-Rats, Inbred Strains, pubmed-meshheading:3403563-Spleen, pubmed-meshheading:3403563-Thiocyanates
pubmed:year
1988
pubmed:articleTitle
Nuclear lamina heterogeneity in mammalian cells. Differential expression of the major lamins and variations in lamin B phosphorylation.
pubmed:affiliation
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.