Linked Life Data

3403537

Source:http://linkedlifedata.com/resource/pubmed/id/3403537

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
1988-9-16
pubmed:abstractText
Vascular smooth muscle cells produce the bulk of connective tissue protein in major arteries. Previously, we demonstrated that collagen synthesis by cultured bovine aortic smooth muscle cells in exponential growth is low and increases as cells approach confluence and growth slows. To evaluate more directly the effects of the proliferative state, subconfluent cultures of smooth muscle cells were incubated in medium supplemented with 0.5% fetal calf serum; under these conditions of growth factor deprivation, cells become quiescent within 72 h. The mRNA levels for type I and III collagens increased markedly within 12 h; at 48 h, they were between 2- and 15-fold higher than in cells growing exponentially. Upon addition of serum to quiescent cultures, cells re-entered the cycle, and collagen mRNA levels decreased. Expression of alpha 1(I), alpha 2(I), and alpha 1(III) mRNAs differed significantly, indicating differential gene regulation. In nuclear run-off assays, changes detected in collagen transcription were insufficient to account for changes in mRNA levels, suggesting additional post-transcriptional sites of control. The inverse response of collagen expression to the proliferative state suggests that exposure of smooth muscle cells in vivo to factors which influence their growth state could have profound effects on the structure of the arterial wall.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11426-30
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Serum deprivation of vascular smooth muscle cells enhances collagen gene expression.
pubmed:affiliation
Department of Biochemistry, Boston University School of Medicine, Massachusetts 02118.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.