Linked Life Data

34024

Source:http://linkedlifedata.com/resource/pubmed/id/34024

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1979-5-23
pubmed:abstractText
A method for rapidly testing large numbers of chemical structures as potential modulators of the interaction between immunoglobulin E (IgE) and its specific receptors on rat peritoneal mast cells is described. IgE, isolated from the ascitic fluid of a transplantable rat IgE immunocytoma, is labeled with iodine-125 under mild conditions employing the Bolton--Hunter reagent. The antibody is incubated with mixed periotoneal cells at 37 degrees, and the cell-bound IgE is separated from unbound label by sedimentation through an 8% sucrose--polymer solution in microsediment tubes. Optimal conditions for the interaction of 3 nM IgE with 3 X 10(5) mast cells in 150 mul are: incubation time, 2 hr; pH, 6.5--7.0; and ionic strength, equivalent to 150 mM NaCl. Mixed peritoneal cells bind IgE with an affinity equal to that of purified mast cells. Human IgE pentapeptide III and several antiallergic agents do not compete with rat IgE in this assay.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3549
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
353-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1979
pubmed:articleTitle
Facile in vitro method for screening inhibitors of IgE binding to mast cells.
pubmed:publicationType
Journal Article, In Vitro