Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1988-9-8
|
| pubmed:abstractText |
To determine whether neutrophil (PMN) dysfunction observed in patients with blunt trauma could be explained by alterations in PMN functional subpopulations and to further study the origins of PMN heterogeneity, we studied PMN subpopulations in 18 patients with severe blunt trauma by using a micropore filter chemotactic assay and a mouse monoclonal antibody (31D8 Mab). A major PMN subpopulation binds 31D8 Mab avidly (31D8 "bright") and depolarizes and responds chemotactically to formyl peptide (fMLP) and C5a; a minor PMN subpopulation binds 31D8 Mab weakly (31D8 "dull") and fails to depolarize and responds poorly to fMLP and C5a. Fourteen patients with trauma had marked alteration of PMN 31D8 expression compared with healthy controls 52% +/- 20% versus 92% +/- 4% bright PMNs, respectively (p less than 0.01). These patients also had significantly decreased PMN chemotaxis and increased band counts compared with controls 30 +/- 10 micron versus 53 +/- 19 micron (p less than 0.01) and 34% +/- 14% versus 6% +/- 1% (p less than 0.01), respectively. Four patients with less-severe injuries had unaltered 31D8 PMN expression and normal PMN chemotaxis. In patients whose band counts exceeded 20%, there was a strong correlation between the number of bands and the percentage of 31D8 dull PMNs. PMNs that weakly express the 31D8 antigen appear to be less mature than PMNs that strongly express the antigen regardless of cell morphology (i.e., bands, multilobed cells). The data suggest that the decreased PMN chemotaxis and increased infection rate in patients with blunt trauma is caused partly by an increase in the number of poorly functioning 31D8 dull PMNs.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C5,
http://linkedlifedata.com/resource/pubmed/chemical/Complement C5a,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogen Peroxide,
http://linkedlifedata.com/resource/pubmed/chemical/N-Formylmethionine...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0022-2143
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
112
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
208-15
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3397624-Adolescent,
pubmed-meshheading:3397624-Adult,
pubmed-meshheading:3397624-Aged,
pubmed-meshheading:3397624-Aged, 80 and over,
pubmed-meshheading:3397624-Antibodies, Monoclonal,
pubmed-meshheading:3397624-Chemotaxis, Leukocyte,
pubmed-meshheading:3397624-Child,
pubmed-meshheading:3397624-Complement C5,
pubmed-meshheading:3397624-Complement C5a,
pubmed-meshheading:3397624-Female,
pubmed-meshheading:3397624-Humans,
pubmed-meshheading:3397624-Hydrogen Peroxide,
pubmed-meshheading:3397624-Knee Prosthesis,
pubmed-meshheading:3397624-Male,
pubmed-meshheading:3397624-Middle Aged,
pubmed-meshheading:3397624-N-Formylmethionine Leucyl-Phenylalanine,
pubmed-meshheading:3397624-Neutrophils,
pubmed-meshheading:3397624-Osteoarthritis,
pubmed-meshheading:3397624-Wounds, Nonpenetrating
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Neutrophil heterogeneity in patients with blunt trauma.
|
| pubmed:affiliation |
Department of Pediatrics, Hartford Hospital, CT 06115.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|