Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1988-8-31
pubmed:abstractText
In vitro cultivation of Borrelia burgdorferi, the etiologic agent of Lyme spirochetosis, allows for the isolation and growth of this bacterium from infected tissues. However, continuous cultivation in modified Kelly medium causes a reduction in the number of detectable plasmids and the loss of infectivity in the white-footed mouse, Peromyscus leucopus. In an unpassaged culture of B. burgdorferi, nine plasmids were present, including seven linear plasmids ranging in size from 49 to 16 kilobases (kb) and two circular plasmids of 27 and 7.6 kb. The 7.6-kb circular and 22-kb linear plasmids were no longer detectable in spirochetes noninfective in white-footed mice, suggesting that a gene(s) encoding for factors responsible for infection may be present on one or more of these extrachromosomal elements. Furthermore, changes in spirochetal proteins and lipopolysaccharide-like material were observed also during early cultivation and may be related to loss of infectivity.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-2437739, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3290239, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3323225, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3356787, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3513648, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3577474, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3603026, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3623705, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3655406, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3969554, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-3988339, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-4008983, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-4069202, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-4472988, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-4860265, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-5557322, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6086042, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6176137, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6187729, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6192088, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6393604, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6490811, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6520220, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6735474, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-6828118, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-7002030, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-7043737, http://linkedlifedata.com/resource/pubmed/commentcorrection/3397175-7130900
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1831-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Changes in infectivity and plasmid profile of the Lyme disease spirochete, Borrelia burgdorferi, as a result of in vitro cultivation.
pubmed:affiliation
Laboratory of Pathobiology, Rocky Mountain Laboratories, National Institue of Allergy and Infectious Diseases, Hamilton, Montana 59840.
pubmed:publicationType
Journal Article