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rdf:type |
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lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0007634,
umls-concept:C0016911,
umls-concept:C0017638,
umls-concept:C0020205,
umls-concept:C0027819,
umls-concept:C0035804,
umls-concept:C0449432,
umls-concept:C0521116,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1533157,
umls-concept:C1548799,
umls-concept:C1705248
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pubmed:dateCreated |
1988-8-24
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pubmed:abstractText |
1. The effect of the lanthanide cation Gd3+ on voltage-dependent calcium currents in neuroblastoma x glioma (NG108-15) cells has been studied using a whole-cell clamp technique. 2. Gd3+ reduced the amplitude of calcium currents. The amount of inhibition produced by Gd3+ was concentration dependent between about 0.5 and 5 microM and reached a maximum at about 10-20 microM. 3. A proportion of the total calcium current was resistant to blockade by Gd3+. 4. Gd3+-resistant calcium current consisted of two components: a rapidly inactivating, 'fast' component which was activated at potentials more positive than about -45 mV, and a long-lasting, 'slow' component which was activated at potentials more positive than about -10 mV. 5. It was possible to isolate the slow component, in the presence of Gd3+, by selectively inactivating the fast component with a brief depolarizing pre-pulse. The fast and slow components of current probably reflect the activity of two subpopulations of calcium channels which are resistant to block by Gd3+. 6. In control conditions inactivation of calcium current could be described by the sum of a fast (tau congruent to 40 ms at +10 mV) and a slow (tau congruent to 800 ms at +10 mV) exponential decay plus a constant. Gd3+ selectively blocked the slowly decaying current. 7. The current blocked by Gd3+ was activated at potentials more positive than about -35 mV and decayed monoexponentially (tau congruent to 800 ms at +10 mV). 8. It is concluded that under the experimental conditions used in the present study calcium currents recorded in NG108-15 cells are made up of at least three components which reflect the activity of three distinct subpopulations of calcium channels, one of which is selectively blocked by Gd3+.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2409515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2410796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2410797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2411625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2411626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2412202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2432604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2443646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2578071,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-2582115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-286336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-301569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-301570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-3945347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-4400110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-4530316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6087159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6092622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6095365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6097675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6131381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6202853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6207437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6261668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6272298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6283360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6287284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6289246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6296372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6303205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6314245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-6330284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3392676-845593
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
398
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
33-47
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3392676-Animals,
pubmed-meshheading:3392676-Calcium,
pubmed-meshheading:3392676-Calcium Channel Blockers,
pubmed-meshheading:3392676-Electrophysiology,
pubmed-meshheading:3392676-Gadolinium,
pubmed-meshheading:3392676-Glioma,
pubmed-meshheading:3392676-Hybrid Cells,
pubmed-meshheading:3392676-Neuroblastoma,
pubmed-meshheading:3392676-Rats,
pubmed-meshheading:3392676-Tumor Cells, Cultured
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pubmed:year |
1988
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pubmed:articleTitle |
Gadolinium selectively blocks a component of calcium current in rodent neuroblastoma x glioma hybrid (NG108-15) cells.
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pubmed:affiliation |
Department of Pharmacology, School of Pharmacy, University of London.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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