Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-8-19
pubmed:abstractText
Structural and functional changes in the rat small intestine following localized hyperthermia were examined. In anaesthetized male Sprague-Dawley rats a 10 cm segment of mid-small intestine was temporarily exteriorized, suspended in a cup containing Krebs-Ringer solution, and either heated at 43.5 degrees C or sham-heated at 38 degrees C for 45 min. The intestinal segments were studied 1, 4, 7, 21 and 42 days later by histopathological examination, determination of wet weight, dry weight and gross segment area, and by measuring absorption of 15 mM D(+)-glucose containing 14C-labelled D(+)-glucose as a tracer. Intestinal glucose transport was assessed by two different techniques: the everted sac method (in vitro) and luminal perfusion-recirculation (in vivo). After 1 day, heated intestinal segments exhibited marked mucosal damage, consisting of loss of epithelial cells and destruction of villi. Re-epithelialization had occurred by day 4, but mucosal architecture remained abnormal throughout the observation period. Hyperthermia caused significant thickening of the intestinal wall: at 4 days the thickening was due to oedema, whereas at 42 days tissue mass per cm2 in heated segments had increased by approximately 53 per cent compared with sham-heated control segments. At 1 day, net glucose transport in vitro in heated segments was reduced to 20 per cent and the serosal/mucosal concentration ratio to 57 per cent of that of control segments. In vivo, glucose transport in heated intestine at 4 days was 45 per cent of that of controls. From 4 days on, glucose transport improved gradually, and at 42 days there was no significant difference between heated and sham-heated animals.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0265-6736
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
527-35
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Morphological and functional recovery of rat small intestine following localized hyperthermia.
pubmed:affiliation
Radiation Research Laboratory, University of Iowa, Iowa City.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't