Linked Life Data

3391240

Source:http://linkedlifedata.com/resource/pubmed/id/3391240

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-8-17
pubmed:abstractText
We have studied the effects of the phorbol ester tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on the contractility, locomotion, morphology, and adhesion of two mammalian fibroblastic cell lines. Using the silicone rubber substratum technique, we have found that the first observable response to the tumor promoter is a rapid weakening of cell contractility (8-15 min). This is followed by gradual morphological changes, characterized by a hyperextension of the cells' leading lamellae, which stretch out to an unlimited degree, and occasionally even detach from the cell bodies. Treated cells also become able to crawl onto hydrophobic substrata which are insufficiently adhesive to support the spreading of untreated fibroblasts. We suggest that both the hyperextension and the ability to spread on nonadhesive surfaces can be explained as consequences of the reduced contractility, and that this reduced contractility may also help to explain the increased invasiveness and loss of anchorage dependence by transformed cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-4827
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-59
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Changes in fibroblast contractility, morphology, and adhesion in response to a phorbol ester tumor promoter.
pubmed:affiliation
Department of Biology, University of North Carolina, Chapel Hill 27599-3280.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.