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pubmed:abstractText |
Synthetic procedures are presented for a new chelator that forms stable and highly fluorescent complexes with Eu3+. This chelator, 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) is synthesized in a high-yield three-step procedure. BCPDA can be covalently incorporated into proteins under relatively mild conditions, and when complexed with Eu3+ forms a fluorescent product that has a lifetime in the range of 0.4 to 0.7 ms. Thus, it is useful for time-resolved fluorescence immunoassay applications.
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