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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-8-11
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pubmed:abstractText |
The ATP content and the kinetics of acrosome reaction of isolated human spermatozoa were investigated during an in vitro culture period of 3 hours in 3 serum-enriched media commonly adopted in IVF and GIFT procedures (Earle solution, Ham F10 and Menezo B2). The ATP concentration in spermatozoa did not change between 1 and 3 hr of incubation and no differences were seen using the three media under investigation. The percentage of acrosome reactions significantly increased during culture, to a similar extent in the three media. The results suggest that each of the three media is equally well suitable for human sperm culture in preparation of IVF and GIFT procedures.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0303-4569
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
20
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
169-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3389542-Acrosome,
pubmed-meshheading:3389542-Adenosine Triphosphate,
pubmed-meshheading:3389542-Culture Media,
pubmed-meshheading:3389542-Humans,
pubmed-meshheading:3389542-Kinetics,
pubmed-meshheading:3389542-Male,
pubmed-meshheading:3389542-Spermatozoa,
pubmed-meshheading:3389542-Time Factors
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pubmed:articleTitle |
ATP-content and kinetics of acrosome reaction in human spermatozoa: influence of various culture media and incubation time.
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pubmed:affiliation |
Dipartimento di Sanità Pubblica e Biologia Cellulare, II Università di Roma Tor Vergata, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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