Linked Life Data

3389516

Source:http://linkedlifedata.com/resource/pubmed/id/3389516

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-7-29
pubmed:abstractText
A routine semiquantitative procedure which permits soluble calcium-binding proteins to be detected following their adsorption to nitrocellulose membrane filters by liquid scintillation counting of specifically bound 45Ca is described. Proteins with high affinity for calcium such as calmodulin and troponin can be detected with a detection threshold of about 2 micrograms per 400 microliter. Modifications to decrease this limit are feasible and are discussed. This technique should allow calcium-binding proteins of unknown function to be assayed during their purification. It was necessary to treat solutions containing 45Ca with chelex-100 in order to prevent loss of calcium binding which occurred as the decay product (Sc3+) accumulated, suggesting that all studies utilizing 45Ca as a tracer should evaluate possible interference by this ion.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
256-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Routine detection of calcium-binding proteins following their adsorption to nitrocellulose membrane filters.
pubmed:affiliation
Department of Anatomy, University of Ottawa Faculty of Health Sciences, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't