Linked Life Data

3389351

Source:http://linkedlifedata.com/resource/pubmed/id/3389351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-7-29
pubmed:abstractText
This study investigated the possibility that the peritoneum is capable of synthesizing phosphatidylcholine (PC), a lubricant surfactant, in an amount similar to that produced by pulmonary alveoli. The synthesis of PC by rat lung (positive control), liver (negative control), and transparent mesentery (test tissue) was determined by in vitro incubation of these tissues in the presence of (methyl-14C) choline chloride for three hours at 37 degrees C in Warburg flasks. All lipid material present in tissue and incubation media was extracted by the Folch technique. Carrier egg PC was added to each sample and total PC was isolated using high performance thin-layer chromatography. The PC fractions were counted for total radioactivity. No statistically significant difference was observed between the mean radioactive value for mesenteric tissue compared with lung tissue. The mean radioactive value for liver when compared with mesenteric and lung tissue was significantly lower (P less than 0.001). Thus, under the conditions of the experiment, we have demonstrated for the first time that peritoneal tissue is capable of synthesizing PC in amounts similar to that produced by the lung. Electron microscopy of transparent mesentery (test tissue) showed that mesothelium constituted the bulk of the cell population. Therefore, it is most likely that the PC that has been detected in peritoneal fluid is produced by the mesothelial cell. These findings are of significant relevance to developing concepts on the role of surfactant phospholipids in the physiology of peritoneal dialysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0272-6386
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Phosphatidylcholine synthesis by peritoneal mesothelium: its implications for peritoneal dialysis.
pubmed:affiliation
Division of Renal Therapy, Baxter Healthcare Corporation, Round Lake, IL 60073-0490.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro