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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1988-7-29
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pubmed:abstractText |
Calcium homeostasis in synaptosomes is altered during ageing. The cytosolic free calcium concentration, [Ca2+]i was determined in synaptosomes and crude synaptosomal fractions from 3- and 24-month-old rats with the fluorescent indicator quin-2. The [Ca2+]i were around two times higher in 24-month-old rats than in adults, both under resting conditions and after K depolarization. This difference was still observed after incubation with an endogenous heavy metal chelator. To avoid the calcium buffering effect of quin-2, [Ca2+]i values were determined with the use of a null-point method and with fura-2. These methods confirmed the increase in [Ca2+]i with age in synaptosomes. The increase in [Ca2+]i in nerve endings may be pathologically important in brain ageing.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzofurans,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
336-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3386880-Aging,
pubmed-meshheading:3386880-Animals,
pubmed-meshheading:3386880-Benzofurans,
pubmed-meshheading:3386880-Brain,
pubmed-meshheading:3386880-Calcium,
pubmed-meshheading:3386880-Cytosol,
pubmed-meshheading:3386880-Fluorescent Dyes,
pubmed-meshheading:3386880-Fura-2,
pubmed-meshheading:3386880-Male,
pubmed-meshheading:3386880-Potassium,
pubmed-meshheading:3386880-Rats,
pubmed-meshheading:3386880-Rats, Inbred Strains,
pubmed-meshheading:3386880-Synaptosomes
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pubmed:year |
1988
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pubmed:articleTitle |
Cytosolic free calcium levels increase with age in rat brain synaptosomes.
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pubmed:affiliation |
Departamento de Biologia Molecular, Universidad Autónoma de Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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