rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1988-7-29
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-126581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-16453477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-16593513,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-16664926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-17757868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-17770799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-2937058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-3012462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-3016642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-3296191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-3561393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-4041012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-6297005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-6353887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-6791577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-6956859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-7116445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-7226224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-7335493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3386631-881736
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0270-7306
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1985-92
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:3386631-Acyltransferases,
pubmed-meshheading:3386631-Amino Acid Sequence,
pubmed-meshheading:3386631-Base Sequence,
pubmed-meshheading:3386631-DNA,
pubmed-meshheading:3386631-Enzyme Induction,
pubmed-meshheading:3386631-Light,
pubmed-meshheading:3386631-Molecular Sequence Data,
pubmed-meshheading:3386631-Plant Proteins,
pubmed-meshheading:3386631-Plants,
pubmed-meshheading:3386631-RNA, Messenger,
pubmed-meshheading:3386631-Transcription, Genetic
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pubmed:year |
1988
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pubmed:articleTitle |
Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.
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pubmed:affiliation |
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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