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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-7-28
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pubmed:abstractText |
In crude membrane fractions of rat pancreatic islets and of RIN-A2-cells, forskolin and NaF stimulated adenylate cyclase activity. Basal and stimulated enzyme activity was approximately 3 to 6 fold higher in membranes of RIN-A2-cells than in membranes of islet cells. In RIN-A2-cells GppNHp and NEM inhibited forskolin-stimulated enzyme activity. The inhibitory effect of GppNHp could be reduced by NEM. It is suggested that the adenylate cyclase system of RIN-A2-cells contains inhibitory and stimulatory N-proteins and that there are critical thiols related to Ni, Ns and/or the catalytic unit. Thus, membrane fractions of RIN-A2-cells may be an appropriate model for studies on the adenylate cyclase system of insulin-producing cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0263-6484
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-5
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:3378316-Adenylate Cyclase,
pubmed-meshheading:3378316-Animals,
pubmed-meshheading:3378316-Cell Membrane,
pubmed-meshheading:3378316-Cells, Cultured,
pubmed-meshheading:3378316-Ethylmaleimide,
pubmed-meshheading:3378316-Forskolin,
pubmed-meshheading:3378316-Guanosine Triphosphate,
pubmed-meshheading:3378316-Guanylyl Imidodiphosphate,
pubmed-meshheading:3378316-Rats,
pubmed-meshheading:3378316-Sodium Fluoride
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pubmed:year |
1988
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pubmed:articleTitle |
Adenylate cyclase in membrane fractions of RIN-A2-cells: studies with forskolin, NaF, GppNHp and NEM.
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pubmed:affiliation |
Department of Pharmacology, University of Tübingen, Federal Republic of Germany.
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pubmed:publicationType |
Journal Article
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