Linked Life Data

3374706

Source:http://linkedlifedata.com/resource/pubmed/id/3374706

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1988-6-27
pubmed:abstractText
The influence of hyperthermia (42 degrees C) on the reversibility of hydroxyurea- and the iron-chelator 2,2'-bipyridine-induced synergistic inhibition of DNA biosynthesis was studied in intact P388 murine lymphocytic leukemia cells in vitro. The cytotoxicity caused by hydroxyurea alone and in combination with bipyridine for 3 h at 37 degrees C and 42 degrees C was found to be completely reversible. However, the inhibition of DNA biosynthesis was irreversible when hydroxyurea- and iron-chelate-treated and washed cells at 37 degrees C were subjected to hyperthermia at 42 degrees C. A potentiation in DNA biosynthesis was also observed when the P388 cells were subjected to hyperthermia along with the combination of hydroxyurea and 2,2'-bipyridine. These effects are probably due to the heat-sensitive nature of the ribonucleotide reductase enzyme, the target site of the effect of hydroxyurea and 2,2'-bipyridine.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0028-2685
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Hyperthermia blocks reversibility of hydroxyurea and bipyridine induced synergistic inhibition of DNA biosynthesis in P388 murine leukemia cells.
pubmed:affiliation
Cancer Research Institute, Tata Memorial Center, Parel, Bombay, India.
pubmed:publicationType
Journal Article