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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1988-7-5
|
| pubmed:abstractText |
Mutagenesis of a diploid human fibroblast strain, KD, with the chemical carcinogen 4 nitroquinolin-1-oxide led to the isolation of stably immortalized neoplastic substrains. Four of these transformed strains, HuT-11, -12, -13, and -14, have been characterized in great detail with regard to morphology and changes in gene expression from the parental KD strain. The HuT-11, -12 and -13 substrains are immortalized and non-tumorigenic, in contrast to HuT-14 which is both immortalized and tumorigenic. The HuT-14 substrain expresses a defective beta-actin as a consequence of a point mutation in 1 of the 2 functional beta-actin alleles. All 4 HuT strains have induced expression of the phosphoprotein plastin and 2 EGF-related polypeptides, and down-regulated expression of the transformation-sensitive tropomyosin isoforms. KD and HuT cells expressing high levels of exogenous mutant beta-actin after gene transfection show morphological alterations. HuT-12 transfectants with excessive mutant beta-actin expression exhibit an elevated tumorigenic potential and tropomyosin-isoform switching characteristic of the tumorigenic HuT-14 strain.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0027-5107
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
199
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
437-47
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:3374511-Actins,
pubmed-meshheading:3374511-Animals,
pubmed-meshheading:3374511-Cell Transformation, Neoplastic,
pubmed-meshheading:3374511-Chromosome Aberrations,
pubmed-meshheading:3374511-Chromosome Banding,
pubmed-meshheading:3374511-Chromosome Disorders,
pubmed-meshheading:3374511-Fibroblasts,
pubmed-meshheading:3374511-Fibrosarcoma,
pubmed-meshheading:3374511-Genes,
pubmed-meshheading:3374511-Humans,
pubmed-meshheading:3374511-Karyotyping,
pubmed-meshheading:3374511-Mice,
pubmed-meshheading:3374511-Mice, Nude,
pubmed-meshheading:3374511-Mutation,
pubmed-meshheading:3374511-Neoplasm Transplantation,
pubmed-meshheading:3374511-Peptides,
pubmed-meshheading:3374511-Transplantation, Heterologous,
pubmed-meshheading:3374511-Tropomyosin
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Macromolecular changes accompanying immortalization and tumorigenic conversion in a human fibroblast model system.
|
| pubmed:affiliation |
Armand Hammer Cancer Research Center, Linus Pauling Institute of Science and Medicine, Palo Alto, CA 94306.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|