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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1988-6-30
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pubmed:abstractText |
Immunization of cattle with a purified Anaplasma marginale major surface protein, AmF36, induced protection against homologous challenge with the Florida isolate. Similarly, immunized cattle were protected from challenge with the antigenically and structurally distinct Washington-O isolate of A. marginale. The degree of protection in AmF36-immunized cattle varied from complete prevention of rickettsemia to significant delay in the onset of rickettsemia compared with control immunized cattle. A single AmF36 vaccinate was not protected against homologous challenge despite development of a strong antibody response. Immunoprecipitation of A. marginale proteins with a monoclonal antibody to AmF36 identified minor molecular size heterogeneity in this protein from different isolates, including the Florida and Washington-O isolates. The apparent molecular size of this surface protein in the Florida isolate was 36 kilodaltons, whereas the analogous proteins in Washington-O and four other isolates of A. marginale from the United States had molecular masses of 33 to 34 kilodaltons. Significantly, the surface-exposed peptides of these proteins appear to be conserved among the different isolates. These results demonstrate the potential of AmF36 as a subunit immunogen for bovine anaplasmosis and indicate a structural basis for its cross-protective ability.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-115917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-14035668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-14035669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-2415453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-2415457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-2443451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-2448258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-2452797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3106214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-320200,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3372021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3551073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3711298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3826850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-3945825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-4031496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-4193922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-596296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6084430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6172996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6203210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6205996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6234364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6361125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6584528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6736645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3372018-6852911
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1526-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:3372018-Anaplasma,
pubmed-meshheading:3372018-Anaplasmosis,
pubmed-meshheading:3372018-Animals,
pubmed-meshheading:3372018-Antibodies, Bacterial,
pubmed-meshheading:3372018-Antigens, Bacterial,
pubmed-meshheading:3372018-Cattle,
pubmed-meshheading:3372018-Cattle Diseases,
pubmed-meshheading:3372018-Membrane Proteins,
pubmed-meshheading:3372018-Molecular Weight
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pubmed:year |
1988
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pubmed:articleTitle |
Immunization of cattle with a 36-kilodalton surface protein induces protection against homologous and heterologous Anaplasma marginale challenge.
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pubmed:affiliation |
Department of Comparative and Experimental Pathology, University of Florida, Gainesville 32610.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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