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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-7-8
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pubmed:abstractText |
Changes in methemoglobin (Met-Hb) concentrations during storage of whole blood and a hemolysate at refrigerated or various freezing temperatures were examined using experimentally prepared blood samples. When whole blood was stored at 3 degrees C, rapid reduction of Met-Hb was observed in the nitrite-treated blood whereas neither reduction nor formation of Met-Hb was observed in the untreated and heated blood within 7 days. When hemolysate was stored at 3 degrees C, Met-Hb concentrations were stable within a few days regardless of the initial values. However, slight autoxidation was observed 7 days after storage in the untreated and heated blood. When whole blood was stored at various freezing temperatures, Met-Hb concentrations were practically stable until at least 30 days at -80 degrees C or -196 degrees C regardless of the initial values, although considerable autoxidation was observed at -30 degrees C especially in the blood containing small amounts of Met-Hb. Based on the results obtained, a new method was devised for long-term storage of whole blood at extremely low temperatures for Met-Hb determinations.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0379-0738
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
37
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
99-104
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:3371807-Blood Preservation,
pubmed-meshheading:3371807-Freezing,
pubmed-meshheading:3371807-Humans,
pubmed-meshheading:3371807-Methemoglobin,
pubmed-meshheading:3371807-Oxidation-Reduction,
pubmed-meshheading:3371807-Reference Values,
pubmed-meshheading:3371807-Spectrophotometry,
pubmed-meshheading:3371807-Time Factors
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pubmed:year |
1988
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pubmed:articleTitle |
Long-term storage of blood samples as whole blood at extremely low temperatures for methemoglobin determination.
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pubmed:affiliation |
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article
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