Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1988-6-27
|
| pubmed:abstractText |
13C-NMR with 13C-enriched taurine [( 13C]taurine) has been utilized to study the formation and reactions of N-chlorotaurine in solution and in human cells. Taurine reacts instantaneously with HOCl at pH 7.0 to form N-chlorotaurine, which is stable in solution by itself. In the presence of alpha-amino acids, a chlorine transfer reaction taken place to produce N-chloroamino acids, which quickly convert to the corresponding aldehydes. [13C]Taurine was incubated with human neutrophils and with cultured human lymphoblastoid cells and 13C-NMR spectra of the whole cell mixtures were acquired in order to examine the formation of N-chlorotaurine from reaction between taurine and the endogenous HOCl produced by myeloperoxidase-catalyzed reactions (Zgliczynski, J.M., et al. (1968) Eur. J. Biochem. 4, 540; Weiss, S.J., et al. (1982) J. Clin. Invest. 70, 598). The presence of N-chlorotaurine in the cells was not detected on the 13C-NMR spectra. On the other hand, N-chloro[13C]taurine incubated with the cells was found to be converted to taurine, which must have been produced by a chlorine transfer reaction of the N-chlorotaurine to other cellular components such as amino acids, peptides or proteins. A 13C-NMR study of taurine uptake in human lymphoblastoid cells indicated that taurine is incorporated into a freely mobile intracellular pool. These results suggest that the presence of abundant taurine in a freely mobile intracellular pool may serve as a buffer in preventing oxidative damage to the cells from attacks by HOCl or other oxidants.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
13
|
| pubmed:volume |
969
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
242-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
13C-NMR study of taurine and chlorotaurine in human cells.
|
| pubmed:affiliation |
New York State Institute for Basic Research in Developmental Disabilities, Staten Island 10314.
|
| pubmed:publicationType |
Journal Article,
In Vitro
|