Linked Life Data

3366570

Source:http://linkedlifedata.com/resource/pubmed/id/3366570

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1988-6-20
pubmed:abstractText
We describe a new technique for the separation and isolation of nonpigmented ciliary body epithelial cells from rabbit. Excised ciliary body is incubated in a medium buffered with EGTA to a free-Ca2+ concentration of 10(-8) M, and the nonpigmented cell layer is separated from the pigmented layer by microdissection under direct visualization. This technique yields intact cells which are greater than 99% nonpigmented. It can be used to produce preparations of nonpigmented cell plasma membrane and of viable whole cells, which may be useful for biochemistry, pharmacology, cell transport studies and tissue culture.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0146-0404
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
817-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Isolation of non-pigmented epithelial cells from rabbit ciliary body.
pubmed:affiliation
Department of Ophthalmology, Jules Stein Eye Institute, UCLA School of Medicine 90024-1771.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't