Linked Life Data

3366513

Source:http://linkedlifedata.com/resource/pubmed/id/3366513

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-6-21
pubmed:abstractText
The effect of purified 2'-5' linked polyadenylated oligomers on the human natural killer (NK) activity has been investigated. Permeabilization of NK cells to ppp(A2'p5')2A did not enhance their NK activity at any concentration tested. Furthermore, concentrations higher than 0.1 mM inhibited their NK activity. Similar results were obtained by pre-incubating non-permeabilized intact cells, indicating an extracellular effect of these compounds on cell membranes, which was further investigated. This inhibition by the oligomers was greater (50% inhibition was obtained with 50 micron), when they were added directly to the NK assay, than when the NK cells were pre-incubated. Similar inhibitory effects were observed with ppp(A2'p5')A and ppp(A2'p5')3A oligomers but not with the dephosphorylated "core" molecules or other nucleotides. The inhibition was completely or partially reversed by phorbol esters and by interferon (IFN), respectively. The inhibition was temperature and extracellular Ca2+ dependent. The oligomers did not inhibit the binding of the effector cells to the target cells. The programming for lysis was the step of the lytic mechanism affected, possibly due to the alteration of the Ca2+ influx into the NK cells observed in presence of the ppp(A2'p5')nA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0192-0561
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Inhibition of natural killer cytotoxicity by extracellular ppp(A2'p5')nA oligonucleotides.
pubmed:affiliation
Department of Microbiology, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't