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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1978-1-27
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pubmed:abstractText |
Fatty acid synthetase was covalently labelled with [14C]palmitic acid from [14C]palmityl-CoA. Tryptic and peptic digestion of the [14C]palmityl enzyme resulted in the formation of radioactive palmityl peptides carrying the long-chain acyl residue both in oxygen-ester and thio-ester linkage. The lipophilic palmityl peptides were purified by column and thin-layer chromatography using organic lolvent systems. Peptides arising from the acyl carrier protein, the condensing enzyme and the palmityl transferase were identified and characterized. The amino acid sequence of a 4'-phosphopant-etheine-containing peptide was established. It comprises 13 residues and shows a high degree of homology with the acyl carrier protein from Escherichia coli. A heptapeptide and an octapeptide from the palmityl transferase active site were partially sequenced. The identical amino acid composition of palmityl transferase and malonyl transferase core peptides is briefly discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
80
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13-23
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:336365-Amino Acid Sequence,
pubmed-meshheading:336365-Amino Acids,
pubmed-meshheading:336365-Binding Sites,
pubmed-meshheading:336365-Chromatography, Ion Exchange,
pubmed-meshheading:336365-Chromatography, Thin Layer,
pubmed-meshheading:336365-Electrophoresis, Paper,
pubmed-meshheading:336365-Fatty Acid Synthetase Complex,
pubmed-meshheading:336365-Oxidation-Reduction,
pubmed-meshheading:336365-Palmitic Acids,
pubmed-meshheading:336365-Palmitoyl Coenzyme A,
pubmed-meshheading:336365-Peptide Fragments,
pubmed-meshheading:336365-Saccharomyces cerevisiae
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pubmed:year |
1977
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pubmed:articleTitle |
The palmityl binding sites of fatty acid synthetase from yeast.
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pubmed:publicationType |
Journal Article
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