Linked Life Data

3362303

Source:http://linkedlifedata.com/resource/pubmed/id/3362303

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1988-6-9
pubmed:abstractText
The effect of colchicine on the hypothalamo-neurohypophysial system of rats drinking 2% NaCl for 4, 6, or 90 days was investigated. Colchicine was injected at time 0 for the 4-day group and 48 h before sacrifice for the 6- and 90-day groups. Each experimental group was divided into two subgroups that were injected with 7 micrograms colchicine into either the cisterna magna or the lateral cerebral ventricle. The hypothalamo-neurohypophysial system was investigated immunocytochemically using an antiserum against both neurophysins (NPs) as primary antibody. The arginine vasopressin content of the neural lobe (NL) of rats salt loaded for 4 days and injected with colchicine on days 0 and 3 was determined by radioimmunoassay. The amount of immunoreactive NPs in the supraoptic nucleus, the paraventricular nucleus, and in the NL was decreased in salt-loaded rats. In salt-loaded rats injected with colchicine into the lateral ventricle, a substantially larger amount of immunoreactive NPs was present in these three areas. However, when colchicine was injected into the cisterna magna, only the supraoptic nucleus appeared loaded with immunoreactive NPs, while NPs were depleted from the paraventricular nucleus. In the NL of rats salt loaded for 90 days immunoreactive NPs were diminished markedly, and axon swellings (Herring bodies) had virtually disappeared. Colchicine treatment of these rats caused an increase of immunoreactive NPs in the NL approaching control values and a reappearance of a large number of axon swellings. Results similar to those obtained with immunohistochemistry for NPs were obtained when determining the arginine vasopressin content of the NL by radioimmunoassay.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0028-3835
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Effects of colchicine on the hypothalamo-neurohypophysial system of chronically salt-loaded rats.
pubmed:affiliation
Instituto de Histología y Patología, Universidad Austral de Chile, Valdivia.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't