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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1988-6-9
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pubmed:abstractText |
Trehalase solubilized with 0.5% Triton X-100 and 0.5% deoxycholate from the brush border membrane of rabbit kidney cortex was all adsorbed on phenyl-Sepharose equilibrated with elution buffer containing no detergents, and all the adsorbed enzyme was eluted in one peak on the addition of 0.5% Triton X-100 to the elution buffer, in contrast to the results reported by Nakano and Sacktor (J. Biochem. 97, 1329-1335 (1985], who separated two forms of trehalase differing in hydrophobicity from rabbit kidney. On concentration of detergent-solubilized extracts, followed by incubation at 37 degrees C, however, there appeared trehalase nonadsorbable on phenyl-Sepharose, i.e. a hydrophilic trehalase. Various protease inhibitors added to the concentrated extracts did not inhibit this conversion at all. The concentration-incubation treatment also increased the proportion of trehalase that interacts with Con A-Sepharose. These results indicate that kidney trehalase that interacts with Con A-Sepharose. These results indicate that kidney trehalase is susceptible to some lytic action of a factor(s) intrinsic to the brush border membrane (limited autolysis), as seen with rabbit intestinal trehalase (Yokota et al., (1986) Biochim. Biophys. Acta 881, 405-414). Therefore, in studies of the molecular form of trehalase (and other proteins) in the brush border membrane of the kidney and intestine where a lot of hydrolases exist, it is very important to take account of limited autolysis which results in some chemical modifications without affecting enzymatic activity.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-924X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
103
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
132-6
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pubmed:dateRevised |
2007-12-19
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pubmed:meshHeading |
pubmed-meshheading:3360754-Animals,
pubmed-meshheading:3360754-Chromatography, Affinity,
pubmed-meshheading:3360754-Chromatography, Gel,
pubmed-meshheading:3360754-Chromatography, Ion Exchange,
pubmed-meshheading:3360754-Kidney Cortex,
pubmed-meshheading:3360754-Microvilli,
pubmed-meshheading:3360754-Rabbits,
pubmed-meshheading:3360754-Solubility,
pubmed-meshheading:3360754-Trehalase
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pubmed:year |
1988
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pubmed:articleTitle |
In vitro loss of hydrophobicity of trehalase from the brush border membrane of rabbit kidney cortex.
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pubmed:affiliation |
Research Institute of Environmental Medicine, Nagoya University, Aichi.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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