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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1988-5-26
|
| pubmed:abstractText |
Coarsely ground Lytta vesicatoria FABRICIUS (meloideae/coleoptera), with a cantharidine content of 0.5-1% was dissolved in ethanol and worked into a powdery solution. 1-2 cm2 large swabs were dissolved in the tincture, and with the help of a Karaya plate and an occlusive dressing was administered to the skin in the antebrachii anterior region. This led to a severe inflammation, which after a further 10 h was accompanied by pemphigus and vesiculation. The vesicular liquid was aspirated, centrifuged, the cells resuspended and coated on a small cover-glass in a petri-dish and incubated. The human macrophages were kept in a moisture-saturated atmosphere with 5% CO2 at a temperature of 37 degrees C. The blisters healed quickly and without scaring after a correct dressing of the wounds was administered. The safety of this method was thus tested on human subjects, at first on the author, and proven successful.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0003-2786
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
165
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-6
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:3358529-Cell Separation,
pubmed-meshheading:3358529-Cells, Cultured,
pubmed-meshheading:3358529-Humans,
pubmed-meshheading:3358529-Macrophage Activation,
pubmed-meshheading:3358529-Macrophages,
pubmed-meshheading:3358529-Microscopy, Electron, Scanning,
pubmed-meshheading:3358529-Skin
|
| pubmed:year |
1988
|
| pubmed:articleTitle |
Human macrophages from skin: I. Isolation and culture.
|
| pubmed:affiliation |
Institut für Anatomie der Medizinischen Universität zu Lübeck, BRD.
|
| pubmed:publicationType |
Journal Article
|