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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1988-5-20
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pubmed:abstractText |
The transforming protein encoded by the v-rel oncogene of avian reticuloendotheliosis virus (REV-T) is a very low copy number molecule in the cytosol of transformed cells. Analysis of cytosolic extracts from a REV-T-transformed lymphoid cell line by gel filtration on Sephacryl S-300 indicated that most of the v-rel oncogene product, pp59v-rel, eluted with an apparent molecular mass of 400 kDa. The size of this complex was confirmed by analysis on a fast-protein liquid chromatography gel filtration column. A 40-kDa cellular protein copurified with pp59v-rel on sequential gel filtration on Sephacryl S-200 and immunoaffinity chromatography with a monoclonal antibody directed against pp59v-rel. The 40-kDa cellular protein could also be immunoprecipitated together with pp59v-rel from cell extracts of [35S]methionine-labeled cells, suggesting that pp59v-rel is complexed with the 40-kDa protein in transformed lymphoid cells. Both the 59- and 40-kDa proteins were phosphorylated when the highly purified preparation containing pp59v-rel was incubated with [gamma-32P]ATP and 10 mM MgCl2 in vitro. The identity of the kinase in the highly purified preparation containing pp59v-rel, however, is unknown. Immune complexes recovered from extracts of REV-T-transformed lymphoid cells labeled with [32P]orthophosphate also contained the 59- and 40-kDa phosphoproteins. These observations suggest that pp59v-rel is complexed with a 40-kDa cellular phosphoprotein to form a 400-kDa heteropolymer in the cytoplasm of transformed lymphoid cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-218111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-219596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-2821682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-2829425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-2867231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-3003751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-3004027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-3004745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-3947347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-4443191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6090694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6100915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6243436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6263007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6267806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6269742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6275117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6283142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6296425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6304524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6312449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6319751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-6325177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3357877-825377
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2479-83
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3357877-Animals,
pubmed-meshheading:3357877-Antibodies, Monoclonal,
pubmed-meshheading:3357877-Cell Transformation, Viral,
pubmed-meshheading:3357877-Lymphocytes,
pubmed-meshheading:3357877-Macromolecular Substances,
pubmed-meshheading:3357877-Molecular Weight,
pubmed-meshheading:3357877-Oncogene Proteins, Viral,
pubmed-meshheading:3357877-Oncogenes,
pubmed-meshheading:3357877-Phosphoproteins,
pubmed-meshheading:3357877-Phosphorylation,
pubmed-meshheading:3357877-Protein Binding,
pubmed-meshheading:3357877-Protein Kinases
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pubmed:year |
1988
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pubmed:articleTitle |
The v-rel oncogene product is complexed to a 40-kDa phosphoprotein in transformed lymphoid cells.
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pubmed:affiliation |
Clayton Foundation Biochemical Institute, University of Texas, Austin 78712.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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