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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1988-5-16
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pubmed:abstractText |
1. The amount of sialoglycopeptide (SGP) mixture released from young (Y) erythrocytes by trypsin treatment was significantly larger than that from old (O) ones. 2. The two main membrane-surface sialoglycopeptides (Y-SGP and O-SGP) were isolated in yields of about 45% and 42% from the mixtures by a combination of Sephadex G-50 superfine gel filtration with DEAE-cellulose chromatography, respectively. Molecular weights of 12,000 and 12,100, respectively, were found for the Y-SGP and the O-SGP. 3. The Y-SGP showed a greater proportion of sialic acid than the O-SGP. The contents of galactose, N-acetylglucosamine and N-acetylgalactosamine of the Y-SGP were significantly lower than those of the O-SGP. 4. Both the SGPs exhibited the same reactivities against the MN reagents, but the reactivities against the lectins of Limulus polyphemus and Phaseolus vulgaris (E) were stronger in the Y-SGP than in the O-SGP. Neither the Y-SGP nor the O-SGP showed the reactivity to Arachis hypogaea (anti-T) lectin.
|
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0305-0491
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
89
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
309-15
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:3356136-Amino Acids,
pubmed-meshheading:3356136-Chromatography, DEAE-Cellulose,
pubmed-meshheading:3356136-Chromatography, Gel,
pubmed-meshheading:3356136-Erythrocyte Aging,
pubmed-meshheading:3356136-Erythrocyte Membrane,
pubmed-meshheading:3356136-Humans,
pubmed-meshheading:3356136-Sialoglycoproteins,
pubmed-meshheading:3356136-Trypsin
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pubmed:year |
1988
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pubmed:articleTitle |
Comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin.
|
pubmed:affiliation |
Department of Legal Medicine, School of Medicine, Keio University, Japan.
|
pubmed:publicationType |
Journal Article,
Comparative Study
|