Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
|
pubmed:dateCreated |
1988-5-10
|
pubmed:abstractText |
We have derivatized a series of 125I-labeled 8-phenylxanthines with photoactive aryl azide groups on the 1- or 3-position of the xanthine ring. A 3-azidophenethyl derivative was found to be optimal for use as an antagonist photoaffinity label for adenosine A1 receptors. Following photoactivation, radioactivity was covalently and specifically incorporated into a 34,000-dalton and, to a lesser extent, into a 24,000-dalton polypeptide of rat brain membranes. Photoincorporation into both polypeptides was competitively inhibited by adenosine analogues with a potency order typical of adenosine A1 receptors, but the 24,000-dalton polypeptide bound both agonists and antagonists with lower affinity than the 34,000-dalton polypeptide. Specific photolabeling of receptors in brain membranes of rat, guinea pig, dog, and cow did not show any variation in the 34,000-dalton adenosine receptor binding subunit. The adenosine agonist photoaffinity label [125I]N6-(4-azido-3-iodobenzyl)adenosine also specifically photolabeled the 34,000-dalton polypeptide, but photoincorporation of the agonist was less efficient than the antagonist and, unlike the antagonist, was greatly reduced by guanosine 5'-(beta,gamma-imidotriphosphate). The results indicate that the antagonist photoaffinity label may be more useful than agonists particularly for labeling uncoupled receptors.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Affinity Labels,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic
|
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0022-2623
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
31
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
752-6
|
pubmed:dateRevised |
2007-11-14
|
pubmed:meshHeading |
pubmed-meshheading:3351851-Affinity Labels,
pubmed-meshheading:3351851-Animals,
pubmed-meshheading:3351851-Azides,
pubmed-meshheading:3351851-Brain,
pubmed-meshheading:3351851-Cattle,
pubmed-meshheading:3351851-Chromatography, High Pressure Liquid,
pubmed-meshheading:3351851-Dogs,
pubmed-meshheading:3351851-Guinea Pigs,
pubmed-meshheading:3351851-Iodine Radioisotopes,
pubmed-meshheading:3351851-Isotope Labeling,
pubmed-meshheading:3351851-Membranes,
pubmed-meshheading:3351851-Molecular Weight,
pubmed-meshheading:3351851-Photochemistry,
pubmed-meshheading:3351851-Purines,
pubmed-meshheading:3351851-Rats,
pubmed-meshheading:3351851-Receptors, Purinergic,
pubmed-meshheading:3351851-Structure-Activity Relationship
|
pubmed:year |
1988
|
pubmed:articleTitle |
Photoaffinity labeling adenosine A1 receptors with an antagonist 125I-labeled aryl azide derivative of 8-phenylxanthine.
|
pubmed:affiliation |
Department of Physiology, University of Virginia School of Medicine, Charlottesville 22908.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|