Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1977-12-29
pubmed:abstractText
A bacteriophage T5 mutant has been isolated that is completely deficient in the induction of deoxynucleoside 5'-monophosphatase activity during infection of Escherichia coli F. The mutant bacteriophage has been shown to be deficient in the excretion of the final products of DNA degradation during infection of E. coli F, and about 30% of the host DNA's thymine residues were reinocorporated into phage DNA. During infection with this mutant, host DNA degradation to trichloroacetic acid-soluble products was normal, host DNA synthesis was shut off normally, and second-step transfer was not delayed. However, induction of early phage enzymes and production of DNA and phage were delayed by 5 to 15 min but eventually reached normal levels. The mutant's phenotype strongly suggests that the enzyme's role is to act at the final stage in the T5-induced system of host DNA degradation by hydrolyzing deoxynucleoside 5'-monophosphates to deoxynucleosides and free phosphate; failure to do this may delay expression of the second-step-transfer DNA.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-1106951, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-1202250, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-13096499, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-14462207, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-163355, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-16789131, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-176472, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-21305, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-325230, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4285470, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4554465, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4571806, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4583885, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4879238, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4889472, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4920825, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-4935284, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-5136582, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-5656373, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-5663335, http://linkedlifedata.com/resource/pubmed/commentcorrection/335083-770228
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
642-50
pubmed:dateRevised
2010-9-2
pubmed:meshHeading
pubmed:year
1977
pubmed:articleTitle
Isolation and characterization of a bacteriophage T5 mutant deficient in deoxynucleoside 5'-monophosphatase activity.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.