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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1988-5-11
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pubmed:abstractText |
The tubulin molecule consists of an alpha- and a beta-subunit, each of which exists in several isotypic forms. It has been previously shown that one of the isotypes of neuroblastoma beta-tubulin is phosphorylated at a serine residue in vivo [(1985) J. Cell Biol. 100, 764-774]. Here we identify the phosphorylated isotype as beta 2 (type III). Moreover, the large size of the phosphorylated tryptic peptide and sequence comparisons of vertebrate beta-tubulins suggest that one of the two serines in positions 444 and 446 is the phosphorylated residue. Our results raise the possibility that beta 2-tubulin differs functionally from the other beta-tubulin isotypes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
230
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
142-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:3350148-Cell Differentiation,
pubmed-meshheading:3350148-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3350148-Neuroblastoma,
pubmed-meshheading:3350148-Peptide Fragments,
pubmed-meshheading:3350148-Phosphates,
pubmed-meshheading:3350148-Phosphorylation,
pubmed-meshheading:3350148-Phosphoserine,
pubmed-meshheading:3350148-Protein Kinases,
pubmed-meshheading:3350148-Trypsin,
pubmed-meshheading:3350148-Tubulin,
pubmed-meshheading:3350148-Tumor Cells, Cultured
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pubmed:year |
1988
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pubmed:articleTitle |
Identification of the phosphorylated beta-tubulin isotype in differentiated neuroblastoma cells.
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pubmed:affiliation |
Department of Biochemistry, University of Texas, San Antonio 78284.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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